FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell52.3520000009561b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell52.3520000009561b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077412
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA7800.027.1134995
TCCGATC42950.026.4009573
TTCCGAT43250.026.1658122
CCGATCT43700.025.9478534
CTTCCGA44650.025.5469381
CGATCTG20450.025.3038655
GAGACGT13250.023.4323338
GACGTGT14150.021.1469410
CGATCTT7500.020.9985395
CGTCGAC654.2307973E-420.77578542
CCGTCGA654.234236E-420.77328741
GGGAGAC15350.020.6663156
GGAGACG15550.020.5451977
TTAGCGA550.0039380820.45410727
ACGTGTG14350.020.22507511
AGACGTG15000.020.0986029
CGTGTGC16250.018.8294612
CGATCTC14250.017.3672145
TGCGACG800.001712269416.87382710
ATTCGCG952.82119E-416.57859230
Produced by FastQC (version 0.11.2)