FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell51.352000000955f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell51.352000000955f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144764
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG20650.024.7313235
CGATCTA7650.024.4094625
TCCGATC45300.024.0374683
CCGATCT45450.023.9581384
TTCCGAT45600.023.8793282
GACGCGT702.7034504E-522.50102837
CTTCCGA48950.022.4289361
GGGAGAC19100.021.673356
GAGACGT18150.021.5682138
GGAGACG19700.020.213837
GACGTGT18550.019.76902410
ACGTGTG18400.019.56337411
CGATCTT7200.018.7482325
GTACGCG600.006515839618.748233
AGACGTG19750.018.567879
CGTGTGC21750.017.27423512
CTGTGCG1701.8353603E-917.204269
CGATCTC15550.016.9276645
GCGACGT1451.8096453E-615.51794837
CGGGAGA4950.015.4530885
Produced by FastQC (version 0.11.2)