FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell50.352000000955d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell50.352000000955d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3412682
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA70570.20678750613154112No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC43690.1280224761639086No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT37970.11126146532258208No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG37290.1092688976001866No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT35710.10463910789226773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC60700.024.6846966
GGAGACG62550.023.6668687
GAGACGT62300.022.642368
CGATCTG42550.022.1013585
GACGTGT61100.021.54055410
CCGATCT98950.021.3497124
AGACGTG62650.021.151279
TCCGATC100100.021.1493853
ACGTGTG61050.021.11597611
TTCCGAT100600.021.0666332
CGATCTA16750.020.8189645
CTCGCGA654.2411487E-420.76882628
CGGGAGA12250.019.8348945
CGATCTT16350.019.8146745
CGTGTGC67500.019.4315312
GCGCGTC706.9848006E-419.2867535
CTTCCGA112850.019.1389561
ATCTGCG4150.018.9741447
GACGTTT5300.018.67751110
AGGGAGA34500.017.9331195
Produced by FastQC (version 0.11.2)