FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell5.3520000009503b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell5.3520000009503b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2090203
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA31890.15256891316297985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC35550.030.3132653
TTCCGAT35700.030.3119352
CTTCCGA36950.029.5909331
CGATCTG17750.029.1525575
CCGATCT37050.028.8438074
CGATCTA6000.027.7477445
CGATCTT7600.022.498175
CGATCTC10850.019.9062165
TCTTCCG14600.019.5703265
TCGACGG600.00650001718.75610444
TATATCG1354.2811735E-818.331845
GCTCTTC17250.016.9547393
GTATTAG8200.016.187321
TATACAG6750.015.6653935
CGGTACC3450.015.65764345
GATCTGG12450.015.1794886
GGGGTTA6700.015.1107116
ATCTACG1654.208432E-714.998787
GACTCGT900.003757323114.998788
TTAACGG3500.014.78592735
Produced by FastQC (version 0.11.2)