HT5LJBCXY_l01n02_mock_cell49.352000000955bc.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HT5LJBCXY_l01n02_mock_cell49.352000000955bc.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	387104
Sequences flagged as poor quality	0
Sequence length	51
%GC	51

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2117	0.5468814582127801	No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCT	740	0.19116309828883193	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACG	701	0.1810882863519881	No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT	621	0.16042200545589816	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACA	582	0.15034719351905432	No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCT	562	0.1451806232950318	No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	402	0.10384806150285195	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACTGG	20	7.035637E-4	44.98941	1
TTTGCTA	20	7.035637E-4	44.98941	14
CGGCACT	50	1.0695658E-9	40.532352	44
GGCACTC	80	0.0	39.406452	45
TTAGACG	40	3.4461846E-7	39.380993	36
ACGTATC	35	6.203447E-6	38.602238	40
ACGGGAT	35	6.2476447E-6	38.56235	4
TCGGCTC	80	0.0	36.591705	43
CGCATGC	50	4.8305083E-8	36.028755	41
TATCCGC	25	0.0020971491	36.028755	43
GAGCGGT	50	4.858157E-8	36.00548	37
TCGCCTG	50	4.8692527E-8	35.996178	29
AACTTCT	25	0.0021064836	35.996178	26
CGCACTG	25	0.0021064836	35.996178	24
TGGTATA	50	4.8692527E-8	35.996178	34
CAGTTGG	25	0.0021078198	35.991528	1
AACTCCC	25	0.0021078198	35.991528	7
ACGCATG	70	2.0008883E-11	35.385384	40
CCGTCTT	45	8.62603E-7	35.027958	40
CTTAGAC	45	8.678544E-7	35.000805	35