FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell48.35200000095593.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell48.35200000095593.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3138635
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA92120.29350338602609094No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG58380.18600442549069898No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC55360.17638240827620924No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT46580.14840846418905032No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT46400.14783496647427943No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA39040.12438528213697994No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT37790.12040265911773748No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC36790.11721656070234354No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC36600.11661120200341868No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTA36150.1151774577164914No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC35940.11450837704925867No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATA32080.10221003716583803No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA31510.10039396106906347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGACG26900.023.667927
CGATCTT10950.023.2161545
CCGATCT59800.023.023394
TTCCGAT59550.022.9689332
CGATCTG28250.022.9350665
TCCGATC59800.022.910533
GGGAGAC28950.022.6136346
GAGACGT26900.021.828018
GACGTGT25300.021.69677710
AGACGTG25650.021.2253049
CTTCCGA66000.021.0651021
CGATCTA10000.020.9222745
ACGTGTG25600.020.91524311
CGTGTGC29150.019.52544612
CGGTACT11800.019.07078238
GGTACTA12250.018.55481139
TTGCGGT13000.018.17331535
CCTTGCG12700.017.71704933
TGCGGTA16150.017.41647536
CTTGCGG12950.017.375334
Produced by FastQC (version 0.11.2)