FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell47.35200000095579.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell47.35200000095579.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3582815
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA91440.2552183129745744No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC55100.1537896877176187No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG53340.14887734923516843No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT47320.1320749187440602No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT40140.11203481061679153No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA39410.10999730658713888No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT37110.10357777334302776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG401.5596204E-533.75589838
CGATCTG32950.024.9887545
TTCCGAT75150.024.068332
ACGCGTC751.694887E-624.0072140
CCGATCT75850.023.9055334
TCCGATC75750.023.8776913
CTTCCGA79700.022.5813851
GGAGACG32700.022.2903147
CGATCTT14250.022.25995
GGGAGAC34000.021.9673716
GACGCGT1459.094947E-1221.72884239
GAGACGT31900.021.6504348
TACGCGT654.2344522E-420.77372739
CGATCTA12050.020.7231065
GACGTGT31400.020.56227710
AGACGTG31800.020.303639
ACGTGTG32850.019.5176911
ACGCGTG1354.2649845E-818.33883940
CGGTACC7300.018.19293245
CCGTCGA1507.2523108E-918.00540741
Produced by FastQC (version 0.11.2)