HT5LJBCXY_l01n02_mock_cell46.35200000095550.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HT5LJBCXY_l01n02_mock_cell46.35200000095550.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38917
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	270	0.6937842074157823	No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCT	132	0.33918339029216027	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACG	113	0.2903615386591978	No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACA	95	0.2441092581648123	No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCT	88	0.2261222601947735	No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT	62	0.15931341059177223	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46	0.11820027237454069	No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43	0.11049155895880977	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGT	20	6.9494755E-4	45.000004	39
ATACGCG	20	6.9494755E-4	45.000004	38
CGTCGGC	20	6.9494755E-4	45.000004	41
CTGATCT	30	1.1197752E-4	37.499996	23
TGCTCTC	30	1.1197752E-4	37.499996	16
CGGGAGC	30	1.1197752E-4	37.499996	34
TACGGGA	25	0.002082234	36.0	3
CCGCAGG	25	0.002082234	36.0	1
CCGTTCT	45	8.4163E-7	35.0	23
ACAGGGA	45	8.4163E-7	35.0	3
CGAGGGA	40	1.5223097E-5	33.750004	3
TTCCGTT	35	2.770914E-4	32.142857	21
TTCTGAT	35	2.770914E-4	32.142857	21
TCTCGTC	35	2.770914E-4	32.142857	27
TCCGTTC	35	2.770914E-4	32.142857	22
GATCTCT	50	1.9173804E-6	31.5	25
TCTTCTG	80	9.276846E-11	30.937502	19
GTGCACA	45	3.4058266E-5	30.0	9
CTCTTCT	90	1.0913936E-11	30.0	18
GGGAGCA	30	0.005087065	29.999998	7