FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell45.35200000095536.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell45.35200000095536.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409033
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA17660.12533418308868566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG10450.028.4162125
TTCCGAT26400.027.0114842
TCCGATC26200.026.9600983
CCGATCT26200.026.7883784
CTTCCGA28500.025.1790281
CGATCTA5250.022.2819185
CGATCTT5700.022.1014985
CCGTACG550.003941667720.4503362
ACCGGGC1009.072719E-720.2465528
CGATCTC8800.018.6615945
ATCTGCG1257.640971E-616.1972397
CGCTAGA2650.016.13505437
TACGACG2101.07320375E-1016.0686915
TAACACG850.00256482115.88303132
TATCGCC1156.781772E-515.65284231
TTGTCGC2202.1645974E-1015.34374137
ACGTGCG900.003751644915.00170436
CTATCGC1351.5814167E-515.0006430
GATCTGC5700.014.9974456
TCTATAC2701.8189894E-1214.9969143
Produced by FastQC (version 0.11.2)