FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell44.3520000009551c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell44.3520000009551c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479736
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA11030.22991812163356512No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG7310.15237547317691397No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC6350.13236446712358463No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT5310.11068587723247786No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC250.002107037235.99599512
CGCTAGA352.8180573E-432.14263537
ACCGGCC352.8197817E-432.1392868
CAGCGTT300.00514710129.9966669
CGGTACC755.6081262E-827.00825745
ATTTCGT507.150193E-526.99981128
GACGTTG450.001225562224.99982630
CACCGCG551.3647112E-424.55040241
AAGCACG551.3666444E-424.54528435
CGATCTT1800.023.747365
TTCCGAT10650.023.6593422
TCCGATC10850.023.2232233
GAGACGT4950.023.1792418
CCGATCT10700.023.1282714
GGGAGAC5650.023.0947766
AGACGTG4950.022.7247459
GACGTGT5150.022.7159210
ACGCAGC602.462979E-422.5021939
CGTATTG500.002262792822.49984427
CCGCTAG500.002262792822.49984436
Produced by FastQC (version 0.11.2)