FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell43.352000000954f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell43.352000000954f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2678803
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA33520.12513051538317674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC47200.028.0267543
TTCCGAT47300.028.0150642
CGATCTA8250.027.8152965
CCGATCT48000.027.559644
CGATCTG20250.027.5526985
CTTCCGA48450.027.5358451
CGATCTT10700.022.2874095
GACGTGT11800.021.1630610
GAGACGT12700.020.9033458
CGGTACC3600.018.76101345
GGAGACG15200.018.649387
ACGTGTG12650.018.49610511
CGATCTC16900.017.8383875
AGACGTG14050.017.6138329
GGGAGAC16900.017.4390186
GTCTATA3400.017.2040981
ACCGCCT3950.017.08683612
CGTCGTA2700.015.83169310
TAACGCC3900.015.5753084
TACCGTC2750.015.5438417
Produced by FastQC (version 0.11.2)