FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell42.352000000954d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell42.352000000954d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2207004
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG20950.026.2014265
TCCGATC46100.025.5711043
CCGATCT46500.025.3511354
TTCCGAT46650.025.2213972
CTTCCGA50400.023.7018971
CGATCTT9150.021.1444535
CGATCTA7600.021.0166685
GAGACGT17450.020.6273618
GACGTGT17250.019.5623610
ACGTGTG17000.019.05604211
GGAGACG19650.019.0048587
GGGAGAC20600.018.674466
AGACGTG18250.018.4904529
CGATCTC16150.018.108815
GTATTAA5850.017.6897241
CGTGTGC20300.016.95565212
GTATATA7200.015.9351731
ATAATAC5300.015.7052543
GTATTAG6500.015.2285461
GATCTGC11850.014.8079636
Produced by FastQC (version 0.11.2)