FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell41.352000000954bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell41.352000000954bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979002
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA21350.21807922762159831No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC12700.1297239433627306No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG11160.11399363841953336No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT10880.11113358297531568No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT10300.10520918241229334No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA10010.10224698213078216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG554.1649164E-628.63564922
TTCCGAT23250.025.8354262
CCGATCT23200.025.7941324
TCCGATC23400.025.7659573
CGTTTGC1058.54925E-1125.71101612
GGAGACG8500.025.6732067
CGATCTG9950.025.3234145
CGGGTAT801.0547592E-725.3092815
GGGAGAC8900.025.024916
GTATACG450.001226237925.00065436
GAGACGT8200.024.966348
CGTAGCG651.5249521E-524.2276915
CTTCCGA25400.023.7371391
CGATCTT4750.023.6812025
CGATCTA2850.023.68125
CGGTACC1550.023.2347245
GATCTCG2950.022.8784486
AATCGGG903.2866956E-722.49943723
GACGTGT8300.022.49713910
ACGTTTG1156.7757355E-921.51900311
Produced by FastQC (version 0.11.2)