FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell40.35200000095494.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell40.35200000095494.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2348058
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA31320.1333868243459063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT50450.025.5508082
TCCGATC51100.025.2698213
CGATCTG24250.024.9557275
CCGATCT52100.024.9143354
CGATCTA9200.024.4535485
CTTCCGA55350.023.532721
TCCGTAC1003.6548954E-822.4963051
CGATCTT9800.022.26775
GGGAGAC19650.018.7763446
GTACTAG1608.1854523E-1018.2782481
GTATTAA6100.018.0708031
GTATTAG8700.017.8418981
GAGACGT19450.017.3500758
GGAGACG20750.017.3472887
CGATCTC15350.016.8546285
GACGTGT18450.016.70528610
CGGTACC2600.016.45151945
ACGTGTG19400.015.88724311
CGCGTGT850.002561027215.88718841
CCGTCGG3000.015.75479440
Produced by FastQC (version 0.11.2)