FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell4.35200000095004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell4.35200000095004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2388398
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA39360.16479665449393274No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC24050.10069511027894013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT40000.029.6410872
TCCGATC40200.029.493623
CTTCCGA40400.029.458991
CCGATCT40900.028.933834
CGATCTG18500.028.5785165
CGATCTA7150.027.6898215
CGATCTC12450.023.853285
CCTATCG550.003939810720.452718
CGATCTT8350.019.6688945
GTATTAG10000.017.5484241
TCTTCCG16300.017.3910775
GTATTAA7450.017.2132191
GAATCGG2105.456968E-1217.14167622
GCGGAAT2005.0931703E-1116.87383819
TTGCGGT5800.016.68128435
CGGTACT5550.016.62256438
CCTTGCG5700.016.57919533
CGGTACC3400.016.5509545
TAGCGGA2600.016.44117517
GTACGCG1104.641955E-516.36319427
Produced by FastQC (version 0.11.2)