FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell39.3520000009547a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell39.3520000009547a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249702
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA30570.13588466383547687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC47450.026.7406923
CGATCTG21800.026.4188085
TTCCGAT48050.026.406782
CCGATCT48250.026.1574444
CGATCTA7350.026.0172465
CTTCCGA50600.025.6539971
ACCGTCG3100.021.7715478
GAGACGT17800.021.1069858
GGAGACG19150.021.0287767
CGATCTC15500.020.4652545
GACGTGT16900.020.23422810
GGGAGAC20500.020.1926696
GTAGCGT706.99203E-419.2833718
CGATCTT9800.018.8242445
TACCGTC3000.018.747727
AGACGTG18400.018.7069669
ACGTGTG17650.018.60963611
GACGCGT851.2027256E-418.53416339
GATCTCG5950.017.7709486
ACGCGTG901.8620733E-417.50565540
Produced by FastQC (version 0.11.2)