FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell38.35200000095451.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell38.35200000095451.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3684195
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA76610.2079423048997135No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC45880.12453195338466068No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT38470.1044190114801198No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG38420.10428329662246433No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT38340.10406615285021559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC49750.024.238966
TACGCGT952.1143933E-823.6887739
CGATCTA16050.023.2688755
GGAGACG52650.022.6474767
ATACGCG903.285786E-722.5037238
GCGTCGT602.466635E-422.50310937
GAGACGT50400.022.408648
CGATCTG43600.022.3947165
CCGATCT100100.022.1607864
TCCGATC100550.022.1287333
TTCCGAT101800.021.8791142
GTATACG1401.364242E-1020.8954636
GACGTGT50050.020.63245610
CTTCCGA109300.020.583641
CGGGAGA12200.020.2850075
AGACGTG52250.019.8067789
GACGTTT4550.019.77838910
ACGTGTG51100.019.63614711
CGATCTT18150.017.9735415
CGTGTGC56600.017.84728612
Produced by FastQC (version 0.11.2)