FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell37.35200000095437.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell37.35200000095437.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169854
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA12700.10856055541973612No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA300.00513957730.00920941
CGATCTG11300.025.8818345
TTCCGAT25100.024.2898982
TCCGATC25200.024.1042353
CCGATCT26000.023.276044
CTTCCGA26600.022.5818611
GCGGAAT550.003938355520.45295119
CCGTCGG1051.4413708E-619.29163440
ACTGCGT600.006515098318.7477388
TAATACG750.001111138517.997834
CGGTGCA750.001111138517.997839
TACCGTC1002.0488114E-517.9978287
CCCGCGA1153.4513341E-617.60732520
ACCGTCG1153.452742E-617.6065718
CGATCTC7800.017.594035
CGATCTA4500.017.4978895
CGATCTT5600.017.2747025
GGGAGAC10200.017.2038086
CTCGTTA1053.1157237E-517.14078911
GACGTGT9150.016.96516610
Produced by FastQC (version 0.11.2)