FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell36.3520000009541d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell36.3520000009541d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2714581
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA31680.1167030934055753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC50400.026.6043873
CGATCTG22300.026.5331255
TTCCGAT50750.026.3322492
CCGATCT51450.026.1488954
CGATCTA9400.025.6090495
CTTCCGA56350.024.0347771
CGATCTT9850.021.9266645
GAGACGT15800.020.6465958
CCGTCGA807.550314E-519.6934441
GACGTGT15500.019.15930610
ACGTGTG15600.018.89227311
GGAGACG18250.018.7377857
GGGAGAC19050.018.6594856
AGACGTG16200.017.9148129
CGATCTC17450.017.5345
GTCGACG901.8612444E-417.50689343
CGACGTC1053.112793E-517.1442438
CGTGTGC18550.016.6155312
CGTCGAC1004.1571705E-415.75620642
TATATCG1004.1729322E-415.7483695
Produced by FastQC (version 0.11.2)