FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell35.352000000953e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell35.352000000953e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703770
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA17570.10312424799121947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA5700.027.2324895
TCCGATC34600.027.1127193
CCGATCT35100.026.7264984
TTCCGAT35350.026.4102082
CGATCTG16200.026.3846745
CTTCCGA36200.026.0386581
CGATCTT6000.022.1214645
GACGTGT8800.020.70691710
GAGACGT9450.019.9968058
CGTCGAC807.54092E-519.69591542
ACGTGTG9200.019.56209211
CGATTAC1051.4392808E-619.29565845
CGATCTC11900.019.282635
AGACGTG9650.018.4167469
TTCGCCG1102.2600962E-618.4066917
GGGAGAC11550.017.9192146
GGAGACG11450.017.6827647
TATCGCC1153.4491404E-617.60949933
CGTGTGC10850.017.41657312
CGTATTG4150.016.80552927
Produced by FastQC (version 0.11.2)