FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell34.352000000953d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell34.352000000953d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674640
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA9410.13948179769951383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGC250.002107651735.9959688
TTCCGAT11350.026.5609022
TCCGATC12000.024.9347063
CCGATCT12150.024.6268714
CGATCTG5250.024.4258375
GTCTAAT651.5238602E-524.2280561
CTTCCGA12850.024.1607171
CGATCTA1900.023.6815575
CGAACTA654.2346938E-420.76998329
TCGCCCG550.003937430720.45225510
ACACCGC550.003937430720.4522556
CGATCTC4000.020.247735
GTATTAG2500.019.7977831
GGACGGG1503.4197E-1019.50504540
TAACGGC1051.4432571E-619.28641336
TTAACGG1051.4432571E-619.28641335
GAACGTC1051.445309E-619.2835565
GGCGTCT706.98464E-419.2835548
CCGGTCT706.98464E-419.28355424
GAGACGT3100.018.8688538
Produced by FastQC (version 0.11.2)