FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell33.352000000953be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell33.352000000953be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459124
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGT250.002104656836.00918639
GGGAGAC31100.023.5823356
GAGACGT29600.022.1172378
GGAGACG31850.022.0381327
CGATCTG17800.021.2333685
CGGGAGA6600.021.133795
ACGCGTG754.592254E-521.006840
CGAGGGA1501.4551915E-1120.997443
GACGTTT2400.020.62248610
AGACGTG29850.020.1985449
GACGTGT29750.019.81271210
CCGCTCT1950.019.61366723
ACCGGGA1503.45608E-1019.4976233
ACGTGTG30500.019.1779911
TTCCGAT44750.018.7016322
CCGATCT44850.018.559614
GCTGCGT851.20584926E-418.5271558
TCCGATC45250.018.4949843
TAGACCG750.001111326517.9978075
ACGGGAG2750.017.9978074
Produced by FastQC (version 0.11.2)