FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell32.35200000095395.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell32.35200000095395.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610689
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC46150.026.6660083
TTCCGAT46500.026.4652982
CCGATCT46750.026.3718954
CGATCTG21900.025.8880215
CGATCTA7850.025.22065
CTTCCGA49500.024.9976921
CGATCTT9250.018.9712225
CGATCTC16250.018.5521325
TTAACGG2350.017.2347635
GACGTGT11400.016.97211610
GAGACGT11850.016.8971758
TCGTACG800.001712690216.8734422
ATACCGA1205.1827355E-616.8734426
ATAATAC10300.016.3819833
TACCGTC3350.016.1179147
ACGTGTG13150.015.911077511
GTATTAA6700.015.1105441
CGGTACC3000.015.00551245
TACGACG4450.014.6615675
AGACGTG13150.014.5423829
Produced by FastQC (version 0.11.2)