FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell31.3520000009537b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell31.3520000009537b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687222
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA18730.11101088060729414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA4700.027.2838755
TCCGATC26650.026.2538013
TTCCGAT27000.026.080122
CCGATCT27050.025.6992364
CGATCTG13150.025.1489945
CTTCCGA29200.024.1151811
GAGACGT6500.019.7283388
CGGGTAA1051.4398956E-619.29477544
TACCGTC2400.018.7476927
ACCGTCG2650.018.6769458
GACGGCC4200.017.67639411
CGTCGTA2300.017.60652710
GACGTGT6100.017.33393110
ATTCGTA4300.017.26531424
CGATCTT5750.016.8240155
TTAACGG2159.094947E-1216.74460435
GCATTCG4050.016.66461422
GGAGACG8350.016.43517
GTATTAG5750.016.4327581
AGACGTG6300.016.4265489
Produced by FastQC (version 0.11.2)