FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell30.35200000095352.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell30.35200000095352.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903165
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA2700.031.6621865
TCCGATC14850.027.8748443
TTCCGAT15100.027.4133422
CCGATCT15250.026.848664
CTTCCGA16100.025.7106461
CGATCTG6750.023.330035
TAGCCCG550.00393912120.4516535
CGATCTC5500.019.2245545
CGATCTT3400.019.1884615
GAGACGT4300.018.8345438
CGGCTAA600.0065147618.74734726
GACGTGT4500.017.49752410
ACGTGTG4700.017.23160411
TACGCTA1904.4929038E-1016.5766039
GGAGACG5450.016.5114257
CGCTTCG1257.624758E-616.19950332
CTAGCAC1257.632783E-616.1977083
ATACGCT1956.493792E-1016.1515628
AGACGTG4650.015.9654839
CGTGTGC5650.015.92695112
Produced by FastQC (version 0.11.2)