FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell3.35200000094fec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell3.35200000094fec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097673
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA16900.15396206338317514No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT11430.10412937186211194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC16650.029.3212383
TTCCGAT16750.029.1461852
CTTCCGA16750.029.0118711
CGATCTA2800.028.9255265
CCGATCT17100.028.4180584
CGATCTG7300.027.1204325
CTAGTAC802.9772727E-622.4976313
CGGGCTA1104.0563464E-922.4976315
GGTACCT7600.021.905599
CCGTTAA754.606125E-520.9977916
CGATCTC6650.020.6369255
CGATCTT4150.020.600245
TCTTCCG7700.019.5758615
AATACCG706.988146E-419.2836845
CCGTAGT951.31590405E-518.94710227
CGCCGTA600.006510846318.74973525
GTACCTG9500.018.70855710
GGGTACC8800.018.6628078
TACCTGG9050.018.64444711
ACCTGGT8950.018.60139312
Produced by FastQC (version 0.11.2)