FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell29.35200000095338.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell29.35200000095338.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830625
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA10370.12484574868322047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT18500.027.480752
CGATCTG7450.027.1771725
TCCGATC18750.027.1143423
CCGATCT18900.026.8991474
CGATCTA3450.026.0830825
CTTCCGA19700.025.9209841
CGATCTT4000.022.4966585
TAGCGCG903.2912794E-722.4953044
ACGTGTG5200.021.63140111
GATCTGT3550.021.5460956
GAGACGT5850.021.5352638
GACGTGT5650.021.1030610
CGATCTC6050.020.8233555
ATAATAC3000.020.2457753
GGGAGAC6800.020.1808266
GGAGACG6800.018.8574947
GCGAACT600.006507802818.75060328
TTATACC1202.5000554E-718.7460884
CGCCTAT851.2019675E-418.53335436
GTATTAA1709.276846E-1118.5255451
Produced by FastQC (version 0.11.2)