FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell28.3520000009531e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell28.3520000009531e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457579
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA32610.1326915635265438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC51550.026.7528673
CCGATCT51900.026.3562494
TTCCGAT52300.026.3262042
CGATCTA8700.026.1185745
CGATCTG22750.025.4155275
CTTCCGA54500.025.3460561
CGATCTT9950.023.9679095
GAGACGT15100.021.1572278
GACGTGT14200.020.12161310
CGATCTC16500.019.7711285
GGGAGAC17800.019.3383246
CGGTACC4150.018.98247745
ACGCGTG951.313822E-518.9523940
ACGTGTG14300.018.87959311
GGAGACG18450.018.5350867
TTAACGG4500.018.5000135
GTATTAG10450.018.0842911
AGACGTG16350.017.7508569
CGTGTGC16650.016.6199412
GGGGTTA6550.016.143736
Produced by FastQC (version 0.11.2)