FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell27.352000000952f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell27.352000000952f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768007
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG551.3672351E-424.5498138
CGATCTA11400.024.076265
CGATCTG30000.023.3973885
TCCGATC68250.023.371023
TTCCGAT68900.023.183192
CCGATCT68550.023.1374634
TACGCGT500.002263075422.50399239
GGGAGAC36650.021.9143356
CTTCCGA75650.021.6796681
GACGTGT32250.021.48597310
GAGACGT35300.021.0953798
GGAGACG37850.020.9818067
ACGTGTG32550.020.52766211
AGACGTG34900.019.5322049
CGATCTT12600.019.1050115
CGTGTGC37800.018.45032312
CGGGTAT1608.1854523E-1018.27921115
CGATCTC21950.017.116545
ATTAACG800.00171294616.8731173
ACGCGTG1703.400055E-815.88660640
Produced by FastQC (version 0.11.2)