FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell26.352000000952d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell26.352000000952d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3046543
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA39070.12824371755133607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG30200.026.5196975
TCCGATC64300.025.470163
TTCCGAT64200.025.3346252
CCGATCT64950.025.1810384
CGATCTA10950.024.4488455
CCGTCGA751.6933664E-624.00945741
CTTCCGA68450.023.9588151
GAGACGT23150.022.1569188
GGAGACG25050.021.1050137
GACGTGT22150.020.82119410
CGATCTT13000.020.4203975
GGGAGAC26900.020.3226096
ACGTGTG22750.019.975411
AGACGTG23750.019.5132269
CGTTTGC2450.019.28254712
CGTCGAC851.2014827E-418.5370242
CGTGTGC26600.017.50652312
GATCTGG17750.016.3499666
ATCTGCG4050.016.1089957
TAGTCGG1401.2565743E-616.07116530
Produced by FastQC (version 0.11.2)