FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell24.35200000095296.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell24.35200000095296.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778162
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA22740.12788486088444134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT38500.027.4059052
CGATCTG18400.027.1443485
TCCGATC38950.027.0315173
CCGATCT39550.026.622184
CGATCTA6400.026.3648665
CTTCCGA41400.025.5948521
TCGTTAG500.00226625422.49801825
GAGACGT9650.021.9151698
GACGTGT9300.021.53036110
CGATCTC12750.020.6452395
GGGAGAC11650.020.2771846
ATCTGCG2700.019.1649787
ACGTGTG10200.018.96891811
GGAGACG12200.018.8098187
CGATCTT6700.018.8043145
AGACGTG10650.018.5899129
ATTGCGA750.001106809218.00904845
ACCGTCG2400.017.810938
GTATATA7800.017.3056811
CGTGTGC11500.017.2153912
Produced by FastQC (version 0.11.2)