HT5LJBCXY_l01n02_mock_cell23.3520000009527c.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HT5LJBCXY_l01n02_mock_cell23.3520000009527c.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	92328
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1151	1.2466424053374925	No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCT	283	0.30651589983536953	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACG	276	0.29893423446841694	No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACA	249	0.2696906680530283	No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCT	229	0.2480287670045923	No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT	186	0.20145567975045492	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	178	0.19279091933108047	No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	157	0.1700459232302227	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGT	20	6.9804594E-4	45.024944	39
GACGTCG	20	6.9804594E-4	45.024944	39
CGCCGAC	20	6.9804594E-4	45.024944	38
GTTTTCC	20	7.0178753E-4	44.976162	13
CTTGCTC	30	2.1516316E-6	44.976162	14
GCCACGG	20	7.0178753E-4	44.976162	1
GGCAGAC	40	3.412024E-7	39.375473	35
TGGGCGA	35	6.185528E-6	38.57189	34
ATCTGTT	35	6.185528E-6	38.57189	26
CGATCCG	35	6.185528E-6	38.57189	24
GCTTGCT	35	6.2086146E-6	38.551	13
TCCAAAC	35	6.2086146E-6	38.551	3
ATGCTGT	30	1.124511E-4	37.541145	44
TTCCTAT	30	1.1317229E-4	37.50045	21
TCCGCAC	30	1.1317229E-4	37.50045	22
ATCTGTG	30	1.1317229E-4	37.50045	26
ACGTTTT	60	1.5279511E-10	37.480137	11
TGTGGTC	25	0.0020858312	36.0395	43
ATACGCG	25	0.0020913978	36.019955	38
CTCCACA	25	0.002096976	36.00043	28