FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell22.35200000095246.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell22.35200000095246.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737700
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA15650.21214585875016945No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG10580.14341873390267046No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC9330.12647417649450995No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT8190.11102074013826758No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT7770.10532736884912566No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA7500.10166734444896298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA2400.026.2462445
TCGCCGG450.001227161324.99642417
ATCGCCG551.3688825E-424.54194316
TTCCGAT14450.023.5087452
GGGAGAC6300.023.2109646
CGATCTG6400.023.1998045
CCGATCT14800.022.800794
GACGTGT5350.022.70703110
TCCGATC15000.022.6467593
GAATCGG500.002264837722.49830622
CGTAGCG500.002265730222.4967815
TCGAAAC500.002265730222.4967815
GGAGACG6250.022.3168077
CGGGAGA1650.021.8150625
GATCTCG1156.7702786E-921.518666
AGACGTG5550.021.4834149
GAGACGT5700.021.3127388
CTTCCGA16900.021.0324941
ACGTGTG5350.021.02502811
ATCTCGT550.00393848420.451627
Produced by FastQC (version 0.11.2)