FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell21.35200000095239.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell21.35200000095239.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2918357
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA36100.12369973927110356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG24700.026.5046145
TCCGATC61000.025.8158383
CCGATCT61200.025.5848754
TTCCGAT61700.025.486492
CTTCCGA67550.023.6456381
CGATCTC22000.021.4744515
CGATCTA9600.021.3253235
GAGACGT21000.020.9972428
GGGAGAC23400.020.574226
GATCTCG8900.020.4748366
GGAGACG22650.020.2622387
GACGTGT20700.019.6713310
CGATCTT12500.019.4374475
CGTCGTA4200.018.74753810
ACGTGTG20550.018.7201711
TACCGTC4450.018.7054067
TATAGCG750.001111887417.9973262
ACCGTCG4450.017.6943048
AGACGTG22600.017.5198239
ATACCGT5300.016.97896
Produced by FastQC (version 0.11.2)