FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell20.3520000009521f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell20.3520000009521f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2681675
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG23150.027.7951455
TCCGATC51400.027.5760043
CCGATCT52650.026.9640354
TTCCGAT53150.026.6257152
CTTCCGA54250.026.2102551
CGATCTA9350.025.506345
CGATCTT10850.020.1138825
CGATCTC16250.019.9371475
GGCGTTA3450.018.26613842
TACCGTC3900.017.3065537
GACGTGT9200.016.62933710
GAGACGT10400.016.008568
ACGTGTG9950.015.60198811
CGTTATT4050.015.00516644
GCGTTAT4500.015.00460743
CGTCGTA4250.014.82255310
ATCTGCG4250.014.8225537
ACCGTCG4450.014.6619558
GATCTGA14000.014.6240376
GATCTGC13550.014.6115846
Produced by FastQC (version 0.11.2)