FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell2.35200000094fc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell2.35200000094fc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075047
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA34960.16847811158012324No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT25800.12433453314551429No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC20900.10072061018376934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC29350.029.3574313
CTTCCGA30200.028.8291231
CCGATCT30050.028.748434
TTCCGAT30350.028.3901372
CGATCTA4800.028.122115
CGATCTG13450.028.1011985
CGATCTT6900.022.4976865
CGGGTAC3000.019.4979957
GGTACCT12300.019.0224349
CGTAGCG951.3181081E-518.94496315
CGATCTC12800.018.4551335
ATCTGCG2600.018.171217
GGCGTTA1002.0429492E-518.00509342
CGCAGTA750.001111220217.9985818
GCCAGTA13800.017.7707726
ACCTGGT13550.017.76527412
GTTGATC13650.017.14109417
GGGGTTA9100.017.0586856
ACGGGTA1752.6975613E-916.7125666
TTTCGGG4500.016.50068931
Produced by FastQC (version 0.11.2)