FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell19.352000000951f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell19.352000000951f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722066
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA18950.11004223996060546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC26000.028.9825123
TTCCGAT26450.028.3193362
CCGATCT26900.027.9292144
CGATCTA4300.027.727445
CGATCTG12500.027.354955
CTTCCGA27800.027.2685621
CGGACGT500.002261762322.5056536
CGATCTT5750.021.9095
CGATCTC8900.019.7154625
TTAACGG2001.8189894E-1218.00138335
GAGACGT4250.017.4673638
CGGTACT2850.017.37328738
TACGACG2650.016.9779995
GTATTAG5900.016.3943271
GTATTAA4900.016.067531
GGTACTA3100.015.97453639
ATCTGCG1703.4166078E-815.8794217
TTGCGGT3450.015.65337735
TCTTCCG12650.015.6492855
GACGTGT4350.015.51437710
Produced by FastQC (version 0.11.2)