FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell18.352000000951da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell18.352000000951da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3246457
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA82750.25489325748038555No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC49830.153490405078521No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG44270.1363640424006848No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT43050.13260609951094376No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT42150.12983384655949548No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA35920.1106436955733589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG31400.026.2955285
CCGATCT76550.025.5105614
TTCCGAT77100.025.416122
TCCGATC77350.025.2758053
CTTCCGA83150.023.810361
CGATCTA14500.022.8084015
GGGAGAC29400.021.503276
GAGACGT26650.021.4428278
GGAGACG28800.021.170077
CGATCTT14600.021.1112145
CGATCTC24600.020.3031485
GACGTGT25500.019.93947810
ACGTGTG25100.019.54016711
GATCTCG8750.018.7698296
AGACGTG27650.018.7144999
CGTGTGC28150.017.9025612
CGGTACC6600.017.73504845
TTAACGG6850.017.08094235
GATCTGC16700.016.5704446
TTGCGGT12050.016.24543235
Produced by FastQC (version 0.11.2)