FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell17.352000000951a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell17.352000000951a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3247593
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT60750.028.3674492
TCCGATC60850.028.320833
CCGATCT61350.028.0166764
CGATCTG28000.027.8810565
CTTCCGA62550.027.6590211
CGATCTA10300.025.1187745
CGATCTT11250.022.9976315
CGATCTC21350.019.8106065
TAACGCC4750.017.0508754
GACGTGT12250.016.34525710
GAGACGT13200.016.1915158
TTAGCGA850.002568017515.88096227
ACGTCGT850.002568281315.8807175
CCGGTCG5000.015.29842420
GTCTATA4300.015.1728561
CGCATCG5200.015.14267113
TCTTCCG28450.015.1038925
ACGTGTG12850.015.05681411
GATCTCG8850.014.9984556
ATCTGCG3900.014.9984557
Produced by FastQC (version 0.11.2)