FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell16.3520000009518a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell16.3520000009518a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452449
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA14670.10100182519317373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG803.3796823E-928.13877945
CGATCTG11000.025.974795
CGATCTA4300.025.6370835
TCCGATC24700.024.8660433
TTCCGAT24900.024.6663152
CCGATCT25200.024.5512244
CTTCCGA26050.023.4910351
GAGACGT8750.021.5979338
GACGTGT8300.021.41361410
GTATTAG6200.021.4092431
ACGTGTG8150.021.25563611
AGACGTG8700.021.2048689
GGAGACG10200.019.6304747
ACCGCCT2050.018.65675212
GGGAGAC10850.018.4544546
CGTGTGC9800.018.3655912
CGATCTC8250.017.9982785
TACCCCG1501.4250327E-716.498425
TTATAGT2600.016.4407354
TATAGTG2500.016.198455
Produced by FastQC (version 0.11.2)