FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell15.35200000095160.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell15.35200000095160.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2658548
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA59450.22361830593241122No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG37660.1416562725216923No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC35440.1333058496592877No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT30140.1133701554382317No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT28100.10569679388899504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC34600.024.1233436
GGAGACG34600.023.4731187
CGATCTA12000.023.4351885
TCGACGG702.6942205E-522.5113344
CCGTCGA702.6977475E-522.50751741
TTCCGAT60050.022.0669332
CGATCTG26200.021.7249575
CGGGAGA6950.021.6885095
GAGACGT35300.021.5417848
CCGATCT61750.021.4958554
TCCGATC62000.021.4454653
AGACGTG34700.020.2285529
GACGTGT34550.020.12102510
CTTCCGA69100.019.8931161
CGTAGCG1757.2759576E-1219.28381215
ACGTGTG35150.019.13751411
GCGACGT1709.276846E-1118.53037337
CGATCTT10100.018.4882765
GTATACG750.001110358418.00093336
CGTTTGC3000.017.99822412
Produced by FastQC (version 0.11.2)