FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell14.35200000095154.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell14.35200000095154.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450465
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA31200.2151034323475575No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG19180.1322334561675049No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC19050.13133719186605675No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT16080.11086099974835656No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT15760.10865481069863801No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGT406.1639183E-428.1273539
GGCGTTA755.6292265E-827.0059842
GGGAGAC25000.025.918596
GGAGACG24650.025.556427
GTAGACG450.001226359225.00122536
GAGACGT25600.023.8170628
ATACGCG500.002264249122.50110238
GATACGC602.4688506E-422.4987778
GACGTGT25750.021.406610
AGACGTG25500.021.351789
ACGTGTG25750.020.79498511
CGTGTGC27100.020.25720212
CGGGAGA4450.020.223625
CGATCTG14650.020.1183595
GACGTTT3150.019.99891310
CCGATCT36650.019.7056124
ACGCGTG807.5538795E-519.6911840
TCCGATC37500.019.498943
TTCCGAT37600.019.3274082
TGTACCG706.987234E-419.2846645
Produced by FastQC (version 0.11.2)