FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell13.3520000009512d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell13.3520000009512d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322016
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28800.8943654973665905No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACG5060.15713504919010235No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT4920.1527874391334592Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCT4890.15185580840703566No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.14999254695418862No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.14999254695418862No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCT4300.13353373745403954No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACA3950.12266471231243167No Hit
ATCTTGTACTGAATACATAGGTGCAAGAGGCGAACCAGGGGAACTGAAACA3460.10744807711418067No Hit
GTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTC3320.1031004670575375No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCAT253.8858758E-544.99736428
GTGAGTA207.027745E-444.99736424
GAACATA253.8858758E-544.9973646
GTGCATT501.0713848E-940.516544
TGCATTT501.0713848E-940.516545
GTGGACA403.4368895E-739.3910444
TCTACGT356.2368E-638.5691648
TGCGAGC356.2368E-638.56916434
ACGCATG301.1364106E-437.50944540
CATCTTA301.138491E-437.497838
GTCTACG301.138491E-437.49787
TTGCGAC301.138491E-437.497821
GAGCCGC301.138491E-437.497837
CTGTGAT301.138491E-437.497833
TGATAGT250.002100656536.01466845
TGGACTT504.8428774E-836.01466845
TGGACAT250.002100656536.01466845
CTTCGCA250.002105467335.9978928
GTATTGG250.002105467335.997891
TTAATAC250.002105467335.997893
Produced by FastQC (version 0.11.2)