FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell12.35200000095103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell12.35200000095103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839518
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTAATAAACAG12460.14841849728058243No Hit
GTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTC9460.11268370660307463No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATG9370.11161166288274939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGAGA11050.029.522155
AGACGTT2600.028.5550469
CGGTATA406.161646E-428.1274234
CAGGGAG6700.027.5347634
GACGTTT2750.026.17939210
GGAGACG31550.025.7424647
ACGTTTT801.0540316E-725.31015611
TCCGTTC801.0540316E-725.31015622
CTGGGAG5200.025.0938284
GGGAGAC33100.025.0127876
GAGACGT31550.024.7441438
CGTTTGC2250.023.99777812
GCGACGT1052.3628672E-923.57345637
AAGGGAG3100.023.2236544
GAGTGGT1305.0931703E-1122.50595742
GTTATAC500.002262973222.50193635
CACTATA602.4682874E-422.4979151
CCGTTCT1104.0527084E-922.49791523
TCTGGCC1600.022.4979158
TGGGAGA10000.022.0479585
Produced by FastQC (version 0.11.2)