FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell11.352000000950e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell11.352000000950e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126657
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17041.3453658305502263No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.2013311542196641No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2310.1823823397048722No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT1940.153169583994568Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCT1940.153169583994568No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACG1850.14606377855152106No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCT1400.1105347513362862No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACA1340.10579754770758822No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.1002708101407739No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA302.150182E-645.00000434
TCCGTTA302.150182E-645.00000432
CCGTTAT302.150182E-645.00000433
CTTAACC207.00951E-445.042
ATGGGTC207.00951E-445.012
GTTCTGT207.00951E-445.025
ATAATGG207.00951E-445.09
ATGTGGG207.00951E-445.02
AGCTTAA207.00951E-445.040
ACACATG253.8711107E-545.040
TTACTAT207.00951E-445.044
TGTATAA207.00951E-445.06
AATGGGT207.00951E-445.011
TCGGCTC207.00951E-445.043
TCAGCGA207.00951E-445.017
GCTTAAC207.00951E-445.041
TTAACCG207.00951E-445.043
GTATACG253.8711107E-545.036
TATAATG207.00951E-445.08
TGTCCAC207.00951E-445.043