Basic Statistics
Measure | Value |
---|---|
Filename | HT5LJBCXY_l01n02_mock_cell11.352000000950e8.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 126657 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1704 | 1.3453658305502263 | No Hit |
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 255 | 0.2013311542196641 | No Hit |
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 231 | 0.1823823397048722 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT | 194 | 0.153169583994568 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCT | 194 | 0.153169583994568 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACG | 185 | 0.14606377855152106 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCT | 140 | 0.1105347513362862 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACA | 134 | 0.10579754770758822 | No Hit |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 127 | 0.1002708101407739 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTATA | 30 | 2.150182E-6 | 45.000004 | 34 |
TCCGTTA | 30 | 2.150182E-6 | 45.000004 | 32 |
CCGTTAT | 30 | 2.150182E-6 | 45.000004 | 33 |
CTTAACC | 20 | 7.00951E-4 | 45.0 | 42 |
ATGGGTC | 20 | 7.00951E-4 | 45.0 | 12 |
GTTCTGT | 20 | 7.00951E-4 | 45.0 | 25 |
ATAATGG | 20 | 7.00951E-4 | 45.0 | 9 |
ATGTGGG | 20 | 7.00951E-4 | 45.0 | 2 |
AGCTTAA | 20 | 7.00951E-4 | 45.0 | 40 |
ACACATG | 25 | 3.8711107E-5 | 45.0 | 40 |
TTACTAT | 20 | 7.00951E-4 | 45.0 | 44 |
TGTATAA | 20 | 7.00951E-4 | 45.0 | 6 |
AATGGGT | 20 | 7.00951E-4 | 45.0 | 11 |
TCGGCTC | 20 | 7.00951E-4 | 45.0 | 43 |
TCAGCGA | 20 | 7.00951E-4 | 45.0 | 17 |
GCTTAAC | 20 | 7.00951E-4 | 45.0 | 41 |
TTAACCG | 20 | 7.00951E-4 | 45.0 | 43 |
GTATACG | 25 | 3.8711107E-5 | 45.0 | 36 |
TATAATG | 20 | 7.00951E-4 | 45.0 | 8 |
TGTCCAC | 20 | 7.00951E-4 | 45.0 | 43 |