FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell10.352000000950ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell10.352000000950ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85091
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5050.5934822719206496No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT1980.23269205908968046Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCT1420.16688016358956884No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACG1320.1551280393931203No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCT1120.13162379100022328No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACA1070.12574772890199903No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.12457251648235418No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.11987166680377478No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTCG206.996462E-445.00000430
CGGATAC206.996462E-445.00000435
CTGCACT206.996462E-445.00000428
TCTCACC206.996462E-445.00000427
TGCACTG206.996462E-445.00000429
CTACTCT253.8603153E-545.044
CGCCGAC253.8603153E-545.041
CACCAGG302.1417964E-644.9999961
CGACAGG301.131058E-437.4999961
GCCGACT301.131058E-437.49999642
TCTACTC301.131058E-437.49999643
CTCACCC250.002096179636.028
GGGGTAC250.002096179636.06
GGTATAC250.002096179636.035
GTATACG250.002096179636.036
TATACGC250.002096179636.037
GCGCTGT250.002096179636.039
AGGGCGC250.002096179636.034
GTGCCTT250.002096179636.015
CTCTGGG401.543706E-533.7500042
Produced by FastQC (version 0.11.2)