FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell1.35200000094fa9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell1.35200000094fa9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231355
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA34980.15676573203277827No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT26450.11853783911569427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA6050.030.4927255
TCCGATC31850.029.3134333
CTTCCGA32750.028.7139531
CCGATCT32700.028.6209034
TTCCGAT33250.028.3498272
CGATCTG13250.027.3368035
GGTACCT15800.021.7857289
CGATCTC13450.020.406825
CGATCTT7550.019.9648285
GTTGATC17800.019.08511217
CGGGTAC3200.018.982427
TTAACGG2750.018.81877335
TACCTGG18700.018.76811811
ACCTGGT18300.018.44030812
ACGGGTA1354.2826287E-818.3314446
GTAGCAT19000.018.23659730
GGGTACC18700.018.1665768
CGGTACC2850.018.166245
CCAGTAG19100.018.14030527
GCCAGTA19600.017.9071226
Produced by FastQC (version 0.11.2)