FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell96.35100000095b90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell96.35100000095b90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737773
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA32340.11812520614382567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG27500.022.580915
TCCGATC73500.022.315433
CCGATCT74050.022.11934
TTCCGAT74200.022.0745852
CTTCCGA74250.022.0726181
CGATCTT22250.019.9205475
CGATCTA10650.019.013325
CGATCTC20400.018.6389585
TACGCAA750.001111055817.99927710
TACCGTC3500.017.9992777
GTATTAA6500.017.3171161
GGCGTTA3000.017.24962242
ATACCGC6450.016.04703927
CGGGTAT1552.0700281E-715.967115
CTAGCGT1156.790309E-515.6515464
ATCTGCG3900.015.5762987
CGAGCCG6350.015.23560915
ACCGTCG4000.015.1868898
CGGCGAA1056.040804E-414.99994639
AACGGTC900.003756234714.99967141
Produced by FastQC (version 0.11.2)