FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell95.35100000095b76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell95.35100000095b76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666846
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA55450.3326642053315063No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC34650.20787763236675733No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT31460.18873969160918283No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG29810.17884075673457536No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA27130.16276248675642502No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT23060.13834511406572653No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT22390.13432554657118895No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA19440.11662745088628464No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC19430.11656745734159005No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC18400.11038812223804718No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC16970.10180904534672068No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGG16900.10138909053385856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAACG851.8954597E-723.82256121
CGATCTG17800.021.7400365
CGATCTA7900.021.0744615
TTCCGAT51600.020.8851532
TCCGATC51750.020.7376653
CCGATCT51750.020.6507154
CTTCCGA52400.020.5749341
CGATCTT15950.020.0299325
CGCTCTA807.5697186E-519.68610814
GCGACTC1901.8189894E-1218.94716641
ATCTGCG1651.2332748E-917.726027
GTCGCAT901.8673213E-417.49876444
TCGAATT1701.8317223E-917.20621740
GGGGTTA7900.017.08746
TACGCAA1205.1793995E-616.87380810
GGGTTAG8450.016.507717
GCGGAAT1803.914465E-916.2493419
CTACGGG1257.626717E-616.200838
TAGGGGT8550.016.0514984
GATCTGG8450.015.9752036
Produced by FastQC (version 0.11.2)