FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n01_mock_cell94.35100000095b40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n01_mock_cell94.35100000095b40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2524460
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA49990.19802254739627484No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC29950.11863923373711606No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT26510.10501255714093312No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG25530.1011305388082996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAC709.3180097E-725.71331442
CGATCTA9700.025.5144965
TCCGATC63850.024.8776823
CCGATCT64350.024.6843814
CGATCTG25350.024.673625
TTCCGAT64600.024.6589972
CTTCCGA64950.024.4718591
CGACGCA500.002265853822.49914743
ATCTGCG2400.022.4991467
CGACCGT855.0360704E-621.17650619
CCGTCGA754.606076E-520.9996241
CGATCTT18150.020.8256575
CCGACGC550.003937330620.45498732
ATCGCTA908.260375E-619.99924310
CGATCTC17850.019.2849855
ACCGTAT951.3172801E-518.9466518
GTCGACG851.2055156E-418.5287143
CGCTATC1102.258972E-618.40839412
TATCGCC3800.018.3556631
CGACAAT750.001110562918.0003929
Produced by FastQC (version 0.11.2)